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rat 8 hydroxy deoxyguanosine elisa kit  (Shanghai Korain Biotech Co Ltd)
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Rat 8 Hydroxy Deoxyguanosine Elisa Kit, supplied by Shanghai Korain Biotech Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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PTP promotes the transformation of hematopoiesis to a youthful state by improving the hematopoietic microenvironment in the bone marrow. (A) Representative SA‐β‐gal staining of rat bone marrow (scale bars, 200 μm) (B) Quantification of SA‐β‐gal + cells per area, n = 3/group. <t>(C)</t> <t>TGF‐β1</t> (pg/mL) and TPO (pg/mL) in rat bone marrow, n = 3/group. (D–N) The proportion of lin − cell subsets (HSC, LT‐HSC, ST‐HSC, MPP1, MPP5, MPP2, MPP3, CMP, MPP4, CLP, Pre GM, GMP, Pre MegE, MEP, MKP) in mice bone marrow cells, n = 3–4/group. Statistical analysis: One‐way ANOVA and Bonferroni post‐test. (O) Multiple cytokine profiling quantification of SASP proteins in mice bone marrow, log2‐transformed fold change in mean fluorescence intensity (MFI) compared to the average of D‐gal group, n = 6/group. (P) Representative examples and (Q) quantification of SEC (CD31 low Sca‐1 low ) and AEC (CD31 hi Sca‐1 hi ) in ECs from rat bone marrow and endosteum was detected by flow cytometry, n = 3/group. (R) The third generation of rat bone marrow‐derived BMSCs were cultured for 8 days. Cell viability was measured by MTT assay, n = 5/group. (S–T) Western blot of P16, P21, P27, and P63 expression in rat bone marrow‐derived BMSCs, n = 4/group. (U) Cell cycle analysis of rat bone marrow‐derived BMSCs by flow cytometry, n = 3/group. (V) Quantification of cell cycle phase distribution, n = 3/group. (W) Apoptosis of the third generation of rat bone marrow‐derived BMSCs using Annexin V‐FITC/PI KIT combined with flow cytometry, n = 3/group. Statistical analysis: One‐way ANOVA, Bonferroni post‐test for (D–N) and Tukey post‐test for the others, Data are mean ± SD; error bars denote 95% CI; * p < 0.05, ** p < 0.01, *** p < 0.001.
Tgf β1, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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enzyme linked immunosorbent assay elisa kit  (Shanghai Korain Biotech Co Ltd)
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PTP promotes the transformation of hematopoiesis to a youthful state by improving the hematopoietic microenvironment in the bone marrow. (A) Representative SA‐β‐gal staining of rat bone marrow (scale bars, 200 μm) (B) Quantification of SA‐β‐gal + cells per area, n = 3/group. <t>(C)</t> <t>TGF‐β1</t> (pg/mL) and TPO (pg/mL) in rat bone marrow, n = 3/group. (D–N) The proportion of lin − cell subsets (HSC, LT‐HSC, ST‐HSC, MPP1, MPP5, MPP2, MPP3, CMP, MPP4, CLP, Pre GM, GMP, Pre MegE, MEP, MKP) in mice bone marrow cells, n = 3–4/group. Statistical analysis: One‐way ANOVA and Bonferroni post‐test. (O) Multiple cytokine profiling quantification of SASP proteins in mice bone marrow, log2‐transformed fold change in mean fluorescence intensity (MFI) compared to the average of D‐gal group, n = 6/group. (P) Representative examples and (Q) quantification of SEC (CD31 low Sca‐1 low ) and AEC (CD31 hi Sca‐1 hi ) in ECs from rat bone marrow and endosteum was detected by flow cytometry, n = 3/group. (R) The third generation of rat bone marrow‐derived BMSCs were cultured for 8 days. Cell viability was measured by MTT assay, n = 5/group. (S–T) Western blot of P16, P21, P27, and P63 expression in rat bone marrow‐derived BMSCs, n = 4/group. (U) Cell cycle analysis of rat bone marrow‐derived BMSCs by flow cytometry, n = 3/group. (V) Quantification of cell cycle phase distribution, n = 3/group. (W) Apoptosis of the third generation of rat bone marrow‐derived BMSCs using Annexin V‐FITC/PI KIT combined with flow cytometry, n = 3/group. Statistical analysis: One‐way ANOVA, Bonferroni post‐test for (D–N) and Tukey post‐test for the others, Data are mean ± SD; error bars denote 95% CI; * p < 0.05, ** p < 0.01, *** p < 0.001.
Enzyme Linked Immunosorbent Assay Elisa Kit, supplied by Shanghai Korain Biotech Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elisa detection kit  (Multi Sciences (Lianke) Biotech Co Ltd)
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PTP promotes the transformation of hematopoiesis to a youthful state by improving the hematopoietic microenvironment in the bone marrow. (A) Representative SA‐β‐gal staining of rat bone marrow (scale bars, 200 μm) (B) Quantification of SA‐β‐gal + cells per area, n = 3/group. <t>(C)</t> <t>TGF‐β1</t> (pg/mL) and TPO (pg/mL) in rat bone marrow, n = 3/group. (D–N) The proportion of lin − cell subsets (HSC, LT‐HSC, ST‐HSC, MPP1, MPP5, MPP2, MPP3, CMP, MPP4, CLP, Pre GM, GMP, Pre MegE, MEP, MKP) in mice bone marrow cells, n = 3–4/group. Statistical analysis: One‐way ANOVA and Bonferroni post‐test. (O) Multiple cytokine profiling quantification of SASP proteins in mice bone marrow, log2‐transformed fold change in mean fluorescence intensity (MFI) compared to the average of D‐gal group, n = 6/group. (P) Representative examples and (Q) quantification of SEC (CD31 low Sca‐1 low ) and AEC (CD31 hi Sca‐1 hi ) in ECs from rat bone marrow and endosteum was detected by flow cytometry, n = 3/group. (R) The third generation of rat bone marrow‐derived BMSCs were cultured for 8 days. Cell viability was measured by MTT assay, n = 5/group. (S–T) Western blot of P16, P21, P27, and P63 expression in rat bone marrow‐derived BMSCs, n = 4/group. (U) Cell cycle analysis of rat bone marrow‐derived BMSCs by flow cytometry, n = 3/group. (V) Quantification of cell cycle phase distribution, n = 3/group. (W) Apoptosis of the third generation of rat bone marrow‐derived BMSCs using Annexin V‐FITC/PI KIT combined with flow cytometry, n = 3/group. Statistical analysis: One‐way ANOVA, Bonferroni post‐test for (D–N) and Tukey post‐test for the others, Data are mean ± SD; error bars denote 95% CI; * p < 0.05, ** p < 0.01, *** p < 0.001.
Elisa Detection Kit, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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PTP promotes the transformation of hematopoiesis to a youthful state by improving the hematopoietic microenvironment in the bone marrow. (A) Representative SA‐β‐gal staining of rat bone marrow (scale bars, 200 μm) (B) Quantification of SA‐β‐gal + cells per area, n = 3/group. <t>(C)</t> <t>TGF‐β1</t> (pg/mL) and TPO (pg/mL) in rat bone marrow, n = 3/group. (D–N) The proportion of lin − cell subsets (HSC, LT‐HSC, ST‐HSC, MPP1, MPP5, MPP2, MPP3, CMP, MPP4, CLP, Pre GM, GMP, Pre MegE, MEP, MKP) in mice bone marrow cells, n = 3–4/group. Statistical analysis: One‐way ANOVA and Bonferroni post‐test. (O) Multiple cytokine profiling quantification of SASP proteins in mice bone marrow, log2‐transformed fold change in mean fluorescence intensity (MFI) compared to the average of D‐gal group, n = 6/group. (P) Representative examples and (Q) quantification of SEC (CD31 low Sca‐1 low ) and AEC (CD31 hi Sca‐1 hi ) in ECs from rat bone marrow and endosteum was detected by flow cytometry, n = 3/group. (R) The third generation of rat bone marrow‐derived BMSCs were cultured for 8 days. Cell viability was measured by MTT assay, n = 5/group. (S–T) Western blot of P16, P21, P27, and P63 expression in rat bone marrow‐derived BMSCs, n = 4/group. (U) Cell cycle analysis of rat bone marrow‐derived BMSCs by flow cytometry, n = 3/group. (V) Quantification of cell cycle phase distribution, n = 3/group. (W) Apoptosis of the third generation of rat bone marrow‐derived BMSCs using Annexin V‐FITC/PI KIT combined with flow cytometry, n = 3/group. Statistical analysis: One‐way ANOVA, Bonferroni post‐test for (D–N) and Tukey post‐test for the others, Data are mean ± SD; error bars denote 95% CI; * p < 0.05, ** p < 0.01, *** p < 0.001.
Tnf α, supplied by Cusabio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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il 1β  (Cusabio)
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PTP promotes the transformation of hematopoiesis to a youthful state by improving the hematopoietic microenvironment in the bone marrow. (A) Representative SA‐β‐gal staining of rat bone marrow (scale bars, 200 μm) (B) Quantification of SA‐β‐gal + cells per area, n = 3/group. <t>(C)</t> <t>TGF‐β1</t> (pg/mL) and TPO (pg/mL) in rat bone marrow, n = 3/group. (D–N) The proportion of lin − cell subsets (HSC, LT‐HSC, ST‐HSC, MPP1, MPP5, MPP2, MPP3, CMP, MPP4, CLP, Pre GM, GMP, Pre MegE, MEP, MKP) in mice bone marrow cells, n = 3–4/group. Statistical analysis: One‐way ANOVA and Bonferroni post‐test. (O) Multiple cytokine profiling quantification of SASP proteins in mice bone marrow, log2‐transformed fold change in mean fluorescence intensity (MFI) compared to the average of D‐gal group, n = 6/group. (P) Representative examples and (Q) quantification of SEC (CD31 low Sca‐1 low ) and AEC (CD31 hi Sca‐1 hi ) in ECs from rat bone marrow and endosteum was detected by flow cytometry, n = 3/group. (R) The third generation of rat bone marrow‐derived BMSCs were cultured for 8 days. Cell viability was measured by MTT assay, n = 5/group. (S–T) Western blot of P16, P21, P27, and P63 expression in rat bone marrow‐derived BMSCs, n = 4/group. (U) Cell cycle analysis of rat bone marrow‐derived BMSCs by flow cytometry, n = 3/group. (V) Quantification of cell cycle phase distribution, n = 3/group. (W) Apoptosis of the third generation of rat bone marrow‐derived BMSCs using Annexin V‐FITC/PI KIT combined with flow cytometry, n = 3/group. Statistical analysis: One‐way ANOVA, Bonferroni post‐test for (D–N) and Tukey post‐test for the others, Data are mean ± SD; error bars denote 95% CI; * p < 0.05, ** p < 0.01, *** p < 0.001.
Il 1β, supplied by Cusabio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rat amh (anti-mullerian hormone) elisa kit  (Guangzhou JET Bio-Filtration)
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PTP promotes the transformation of hematopoiesis to a youthful state by improving the hematopoietic microenvironment in the bone marrow. (A) Representative SA‐β‐gal staining of rat bone marrow (scale bars, 200 μm) (B) Quantification of SA‐β‐gal + cells per area, n = 3/group. <t>(C)</t> <t>TGF‐β1</t> (pg/mL) and TPO (pg/mL) in rat bone marrow, n = 3/group. (D–N) The proportion of lin − cell subsets (HSC, LT‐HSC, ST‐HSC, MPP1, MPP5, MPP2, MPP3, CMP, MPP4, CLP, Pre GM, GMP, Pre MegE, MEP, MKP) in mice bone marrow cells, n = 3–4/group. Statistical analysis: One‐way ANOVA and Bonferroni post‐test. (O) Multiple cytokine profiling quantification of SASP proteins in mice bone marrow, log2‐transformed fold change in mean fluorescence intensity (MFI) compared to the average of D‐gal group, n = 6/group. (P) Representative examples and (Q) quantification of SEC (CD31 low Sca‐1 low ) and AEC (CD31 hi Sca‐1 hi ) in ECs from rat bone marrow and endosteum was detected by flow cytometry, n = 3/group. (R) The third generation of rat bone marrow‐derived BMSCs were cultured for 8 days. Cell viability was measured by MTT assay, n = 5/group. (S–T) Western blot of P16, P21, P27, and P63 expression in rat bone marrow‐derived BMSCs, n = 4/group. (U) Cell cycle analysis of rat bone marrow‐derived BMSCs by flow cytometry, n = 3/group. (V) Quantification of cell cycle phase distribution, n = 3/group. (W) Apoptosis of the third generation of rat bone marrow‐derived BMSCs using Annexin V‐FITC/PI KIT combined with flow cytometry, n = 3/group. Statistical analysis: One‐way ANOVA, Bonferroni post‐test for (D–N) and Tukey post‐test for the others, Data are mean ± SD; error bars denote 95% CI; * p < 0.05, ** p < 0.01, *** p < 0.001.
Rat Amh (Anti Mullerian Hormone) Elisa Kit, supplied by Guangzhou JET Bio-Filtration, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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threonine protein kinase akt1 elisa kit  (Shanghai Korain Biotech Co Ltd)
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PTP promotes the transformation of hematopoiesis to a youthful state by improving the hematopoietic microenvironment in the bone marrow. (A) Representative SA‐β‐gal staining of rat bone marrow (scale bars, 200 μm) (B) Quantification of SA‐β‐gal + cells per area, n = 3/group. <t>(C)</t> <t>TGF‐β1</t> (pg/mL) and TPO (pg/mL) in rat bone marrow, n = 3/group. (D–N) The proportion of lin − cell subsets (HSC, LT‐HSC, ST‐HSC, MPP1, MPP5, MPP2, MPP3, CMP, MPP4, CLP, Pre GM, GMP, Pre MegE, MEP, MKP) in mice bone marrow cells, n = 3–4/group. Statistical analysis: One‐way ANOVA and Bonferroni post‐test. (O) Multiple cytokine profiling quantification of SASP proteins in mice bone marrow, log2‐transformed fold change in mean fluorescence intensity (MFI) compared to the average of D‐gal group, n = 6/group. (P) Representative examples and (Q) quantification of SEC (CD31 low Sca‐1 low ) and AEC (CD31 hi Sca‐1 hi ) in ECs from rat bone marrow and endosteum was detected by flow cytometry, n = 3/group. (R) The third generation of rat bone marrow‐derived BMSCs were cultured for 8 days. Cell viability was measured by MTT assay, n = 5/group. (S–T) Western blot of P16, P21, P27, and P63 expression in rat bone marrow‐derived BMSCs, n = 4/group. (U) Cell cycle analysis of rat bone marrow‐derived BMSCs by flow cytometry, n = 3/group. (V) Quantification of cell cycle phase distribution, n = 3/group. (W) Apoptosis of the third generation of rat bone marrow‐derived BMSCs using Annexin V‐FITC/PI KIT combined with flow cytometry, n = 3/group. Statistical analysis: One‐way ANOVA, Bonferroni post‐test for (D–N) and Tukey post‐test for the others, Data are mean ± SD; error bars denote 95% CI; * p < 0.05, ** p < 0.01, *** p < 0.001.
Threonine Protein Kinase Akt1 Elisa Kit, supplied by Shanghai Korain Biotech Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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PTP promotes the transformation of hematopoiesis to a youthful state by improving the hematopoietic microenvironment in the bone marrow. (A) Representative SA‐β‐gal staining of rat bone marrow (scale bars, 200 μm) (B) Quantification of SA‐β‐gal + cells per area, n = 3/group. <t>(C)</t> <t>TGF‐β1</t> (pg/mL) and TPO (pg/mL) in rat bone marrow, n = 3/group. (D–N) The proportion of lin − cell subsets (HSC, LT‐HSC, ST‐HSC, MPP1, MPP5, MPP2, MPP3, CMP, MPP4, CLP, Pre GM, GMP, Pre MegE, MEP, MKP) in mice bone marrow cells, n = 3–4/group. Statistical analysis: One‐way ANOVA and Bonferroni post‐test. (O) Multiple cytokine profiling quantification of SASP proteins in mice bone marrow, log2‐transformed fold change in mean fluorescence intensity (MFI) compared to the average of D‐gal group, n = 6/group. (P) Representative examples and (Q) quantification of SEC (CD31 low Sca‐1 low ) and AEC (CD31 hi Sca‐1 hi ) in ECs from rat bone marrow and endosteum was detected by flow cytometry, n = 3/group. (R) The third generation of rat bone marrow‐derived BMSCs were cultured for 8 days. Cell viability was measured by MTT assay, n = 5/group. (S–T) Western blot of P16, P21, P27, and P63 expression in rat bone marrow‐derived BMSCs, n = 4/group. (U) Cell cycle analysis of rat bone marrow‐derived BMSCs by flow cytometry, n = 3/group. (V) Quantification of cell cycle phase distribution, n = 3/group. (W) Apoptosis of the third generation of rat bone marrow‐derived BMSCs using Annexin V‐FITC/PI KIT combined with flow cytometry, n = 3/group. Statistical analysis: One‐way ANOVA, Bonferroni post‐test for (D–N) and Tukey post‐test for the others, Data are mean ± SD; error bars denote 95% CI; * p < 0.05, ** p < 0.01, *** p < 0.001.
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PTP promotes the transformation of hematopoiesis to a youthful state by improving the hematopoietic microenvironment in the bone marrow. (A) Representative SA‐β‐gal staining of rat bone marrow (scale bars, 200 μm) (B) Quantification of SA‐β‐gal + cells per area, n = 3/group. (C) TGF‐β1 (pg/mL) and TPO (pg/mL) in rat bone marrow, n = 3/group. (D–N) The proportion of lin − cell subsets (HSC, LT‐HSC, ST‐HSC, MPP1, MPP5, MPP2, MPP3, CMP, MPP4, CLP, Pre GM, GMP, Pre MegE, MEP, MKP) in mice bone marrow cells, n = 3–4/group. Statistical analysis: One‐way ANOVA and Bonferroni post‐test. (O) Multiple cytokine profiling quantification of SASP proteins in mice bone marrow, log2‐transformed fold change in mean fluorescence intensity (MFI) compared to the average of D‐gal group, n = 6/group. (P) Representative examples and (Q) quantification of SEC (CD31 low Sca‐1 low ) and AEC (CD31 hi Sca‐1 hi ) in ECs from rat bone marrow and endosteum was detected by flow cytometry, n = 3/group. (R) The third generation of rat bone marrow‐derived BMSCs were cultured for 8 days. Cell viability was measured by MTT assay, n = 5/group. (S–T) Western blot of P16, P21, P27, and P63 expression in rat bone marrow‐derived BMSCs, n = 4/group. (U) Cell cycle analysis of rat bone marrow‐derived BMSCs by flow cytometry, n = 3/group. (V) Quantification of cell cycle phase distribution, n = 3/group. (W) Apoptosis of the third generation of rat bone marrow‐derived BMSCs using Annexin V‐FITC/PI KIT combined with flow cytometry, n = 3/group. Statistical analysis: One‐way ANOVA, Bonferroni post‐test for (D–N) and Tukey post‐test for the others, Data are mean ± SD; error bars denote 95% CI; * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: Aging Cell

Article Title: Periodic Therapeutic Phlebotomy Mitigates Systemic Aging Phenotypes by Promoting Bone Marrow Function

doi: 10.1111/acel.70400

Figure Lengend Snippet: PTP promotes the transformation of hematopoiesis to a youthful state by improving the hematopoietic microenvironment in the bone marrow. (A) Representative SA‐β‐gal staining of rat bone marrow (scale bars, 200 μm) (B) Quantification of SA‐β‐gal + cells per area, n = 3/group. (C) TGF‐β1 (pg/mL) and TPO (pg/mL) in rat bone marrow, n = 3/group. (D–N) The proportion of lin − cell subsets (HSC, LT‐HSC, ST‐HSC, MPP1, MPP5, MPP2, MPP3, CMP, MPP4, CLP, Pre GM, GMP, Pre MegE, MEP, MKP) in mice bone marrow cells, n = 3–4/group. Statistical analysis: One‐way ANOVA and Bonferroni post‐test. (O) Multiple cytokine profiling quantification of SASP proteins in mice bone marrow, log2‐transformed fold change in mean fluorescence intensity (MFI) compared to the average of D‐gal group, n = 6/group. (P) Representative examples and (Q) quantification of SEC (CD31 low Sca‐1 low ) and AEC (CD31 hi Sca‐1 hi ) in ECs from rat bone marrow and endosteum was detected by flow cytometry, n = 3/group. (R) The third generation of rat bone marrow‐derived BMSCs were cultured for 8 days. Cell viability was measured by MTT assay, n = 5/group. (S–T) Western blot of P16, P21, P27, and P63 expression in rat bone marrow‐derived BMSCs, n = 4/group. (U) Cell cycle analysis of rat bone marrow‐derived BMSCs by flow cytometry, n = 3/group. (V) Quantification of cell cycle phase distribution, n = 3/group. (W) Apoptosis of the third generation of rat bone marrow‐derived BMSCs using Annexin V‐FITC/PI KIT combined with flow cytometry, n = 3/group. Statistical analysis: One‐way ANOVA, Bonferroni post‐test for (D–N) and Tukey post‐test for the others, Data are mean ± SD; error bars denote 95% CI; * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The concentration of Klotho (Elabscience, E‐EL‐R2580c), Taurine (Absin, abs580222), TGF‐β1 (Multi Sciences, EK981), TPO (Fine Test, ER0202) was measured in accordance with the protocols outlined in the respective reagent kit manuals.

Techniques: Transformation Assay, Staining, Fluorescence, Flow Cytometry, Derivative Assay, Cell Culture, MTT Assay, Western Blot, Expressing, Cell Cycle Assay